Cell Culture:Article Title: Collagen-Based Products in Wound, Skin, and Health Care
Article Snippet: AlloDerm LifeCell Corporation , Aseptic decellularized human dermal matrix; two forms: freeze-dried or ready to use; contains collagen, elastin, and laminin , Level 1 Level 4 Level 7a , Human skin allograft that has had epidermic and cells removed to prevent tissue rejection , Indicated for soft tissue repair; breast implant reconstruction following mastectomy; skull base reconstruction; periodontal surgeries, dural replacements in neurosurgery, abdominal hernia, head, and neck. , Low levels of fibroblast and blood vessel infiltration compared with other acellular dermal matrices; initial infiltrate of inflammatory macrophages, followed by proliferative phase where endothelial cells can promote revascularization in 7 days (subcutaneous models) , AlloDerm patients showed higher risk of infection and more unplanned operating room visits than other acellular dermal matrices (ADMs) , 96 – 101. .. Apligraf ■ Organogenesis Inc. , Bovine type I collagen matrix cultured with allogeneic male neonatal fibroblasts and keratinocytes , Level 1 Level 3 Level 5 Level 7b , Full-thickness skin equivalent used to treat chronic diabetic and venous ulcers; temporary biological dressing, providing growth factors to the wound , Indicated for use with standard therapeutic compression for the treatment of noninfected partial and full thickness skin ulcers due to venous insufficiency of >1 month duration and which have not adequately responded to conventional ulcer therapy , Apligraf application invokes an inflammatory response which is essential for acute wound healing; possibly reverts the chronic inflammatory response in nonhealing wounds to a beneficial acute inflammatory signaling response; hypothesized mechanisms include Apligraf secreting growth factors and cytokines of the prohealing pathways, stimulating venous leg ulcer (VLU) cells to activate healing pathways, or a combination of the two , The use of Apligraf in conjunction with compression therapy is a safe and effective way to treat VLUs; patients were twice as likely to achieve complete wound closure by 6 months, and over 60% more effective in achieving wound closure than active control , 102 – 112. .. Biodesign (BioD) ■ Cook Biotech. , Acellular porcine small intestinal submucosa (SIS); contains ECM collagen, glycosaminoglycans, proteoglycans, and glycoproteins , Level 1 Level 4 Level 5 Level 7a , Creates an environment that allows cells in the body to secrete growth factors and replicate; as the body heals, SIS is gradually remodeled and integrated into the body, leaving behind organized tissue that provides long-term strength , Implantation to reinforce soft tissue; chordee correction; Peyronie’s disease; urethral repair; anorectal fistula repair reinforcement, rectal prolapse/rectal intussusception repair; ventral and hiatal hernia repair; dura mater graft; duraplasty graft; tympanic membrane perforation closure , Reduces profibrotic gene and protein expression (thrombospondin-1 and fibronectin-1) in fibroblasts; reduces levels of latent transforming growth factor which, in combination with fibronectin-1 downregulation, suggests a mechanism with which BioD scaffolds hinder normal and keloid fibroblast profibrotic responses during tissue repair , Laparoscopic paraesophageal hernia repair patients had long and durable relief of symptoms and improvement in quality of life with SIS; SIS myringoplasty yielded reduced surgical time with no adverse events , 113 – 120.
Control:Article Title: Collagen-Based Products in Wound, Skin, and Health Care
Article Snippet: AlloDerm LifeCell Corporation , Aseptic decellularized human dermal matrix; two forms: freeze-dried or ready to use; contains collagen, elastin, and laminin , Level 1 Level 4 Level 7a , Human skin allograft that has had epidermic and cells removed to prevent tissue rejection , Indicated for soft tissue repair; breast implant reconstruction following mastectomy; skull base reconstruction; periodontal surgeries, dural replacements in neurosurgery, abdominal hernia, head, and neck. , Low levels of fibroblast and blood vessel infiltration compared with other acellular dermal matrices; initial infiltrate of inflammatory macrophages, followed by proliferative phase where endothelial cells can promote revascularization in 7 days (subcutaneous models) , AlloDerm patients showed higher risk of infection and more unplanned operating room visits than other acellular dermal matrices (ADMs) , 96 – 101. .. Apligraf ■ Organogenesis Inc. , Bovine type I collagen matrix cultured with allogeneic male neonatal fibroblasts and keratinocytes , Level 1 Level 3 Level 5 Level 7b , Full-thickness skin equivalent used to treat chronic diabetic and venous ulcers; temporary biological dressing, providing growth factors to the wound , Indicated for use with standard therapeutic compression for the treatment of noninfected partial and full thickness skin ulcers due to venous insufficiency of >1 month duration and which have not adequately responded to conventional ulcer therapy , Apligraf application invokes an inflammatory response which is essential for acute wound healing; possibly reverts the chronic inflammatory response in nonhealing wounds to a beneficial acute inflammatory signaling response; hypothesized mechanisms include Apligraf secreting growth factors and cytokines of the prohealing pathways, stimulating venous leg ulcer (VLU) cells to activate healing pathways, or a combination of the two , The use of Apligraf in conjunction with compression therapy is a safe and effective way to treat VLUs; patients were twice as likely to achieve complete wound closure by 6 months, and over 60% more effective in achieving wound closure than active control , 102 – 112. .. Biodesign (BioD) ■ Cook Biotech. , Acellular porcine small intestinal submucosa (SIS); contains ECM collagen, glycosaminoglycans, proteoglycans, and glycoproteins , Level 1 Level 4 Level 5 Level 7a , Creates an environment that allows cells in the body to secrete growth factors and replicate; as the body heals, SIS is gradually remodeled and integrated into the body, leaving behind organized tissue that provides long-term strength , Implantation to reinforce soft tissue; chordee correction; Peyronie’s disease; urethral repair; anorectal fistula repair reinforcement, rectal prolapse/rectal intussusception repair; ventral and hiatal hernia repair; dura mater graft; duraplasty graft; tympanic membrane perforation closure , Reduces profibrotic gene and protein expression (thrombospondin-1 and fibronectin-1) in fibroblasts; reduces levels of latent transforming growth factor which, in combination with fibronectin-1 downregulation, suggests a mechanism with which BioD scaffolds hinder normal and keloid fibroblast profibrotic responses during tissue repair , Laparoscopic paraesophageal hernia repair patients had long and durable relief of symptoms and improvement in quality of life with SIS; SIS myringoplasty yielded reduced surgical time with no adverse events , 113 – 120.
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